Partial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation
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چکیده
منابع مشابه
Partial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation
1. The ability to phosphorylate ADP during oxidation of NADH by ubiquinone-1 was restored to the NADH-ubiquinone reductase complex by combining the latter with phospholipids and a hydrophobic protein fraction derived from bovine heart mitochondria. 2. Phosphorylation was abolished by rotenone, uncoupling agents, or rutamycin. The efficiency of ATP formation was as high as 0.5 mole per mole of N...
متن کاملPartial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation
1. Passage of submitochondrial particles through a Sephadex cc,lumn activated the adenosine triphosphatase activity 5to IO-fold and subsequent treatment with urea removed virtually all ATPase (coupling factor 1). The resulting submitochondrial particles, exposed sequentially to Sephadex and urea (SU-particles), catalyzed the oxidation of succinate but phosphorylation was insignificant. P: 0 rat...
متن کاملPartial resolution of the enzymes catalyzing oxidative phosphorylation. XVII. Further resolution of the rutamycin-sensitive adenosine triphosphatase.
1. A mitochondrial fraction from beef heart (TU-particles) was shown to confer rutamycin sensitivity to added mitochondrial ATPase (F,). After sonic oscillation at an alkaline pH, the resulting particles (TUA-particles) no longer had this activity. A rutamycin sensitivity factor (FJ, purified from crude preparations of coupling factor 4, restored the capacity of the particles to confer rutamyci...
متن کاملPartial resolution of the enzymes catalyzing oxidative phosphorylation. X. Correlation of morphology and function in submitochondrial particles.
1. Evidence has been presented in this paper which greatly strengthens the suggestion that the spherical particles lining the inner membranes of mitochondria are the morphological representations of coupling factor 1 (ATPase). 2. With tritum-labeled ATPase it was shown that treatment of reconstituted particles with 2 M urea leads to a release of radioactivity which corresponded to the disappear...
متن کاملPartial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation. Iii. a New Coupling Factor Required by Submitochondrial Particles Extracted with Phosphatides.
1. The reaction of mitochondrial adenosine triphosphatase (coupling factor 1) with iodine resulted in rapid loss of ATPase activity, disappearance of sulfhydryl groups, binding of iodine to the protein, and partial dissociation of the molecule. The extent of the changes observed depended on the molar ratio of iodine to protein in the reaction mixture. At a molar ratio of 50, more than 99% of th...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)99410-x